Dr. Mathew John, PhD, currently serving as Scientist D at the Jubilee Centre for Medical Research, Jubilee Mission Medical College & Research Institute. His research interest is focused broadly on Clinical proteomics and metabolomics in pursuit of biomarkers which helps in diagnosis and prognosis of autoimmune and systemic inflammatory diseases. Dr. Mathew john obtained PhD in Neurochemistry from NIMHANS, Bangalore for the thesis entitled Role of Insulin in Alzheimer's disease. He was a recipient of CSIR fellowship for Ph.D. The post doctoral fellowship was done at Rajiv Gandhi center for biotechnology with DBT- RA fellowship. Dr. Mathew John obtained his post graduation in M.Sc Medical Biochemistry from JIPMER, Puducherry. He is a life term member of society of biological chemists(India) and Proteomics Society of India. He has contributed to the areas of Proteomics in Chronic inflammatory diseases and Phytochemistry applications in in- vitro and in- vivo systems. He has more than 40 publications in peer reviewed journals in the field of Clinical proteomics, Immunology & phytochemistry and an active reviewer of Wiley, Springer, Taylor & Francis and Frontiers publications.
Citations- 252
h-index- 08
i10 index- 07
Orcid ID- https://orcid.org/0000-0002-8856-9984
Google Scholar ID- https://scholar.google.com/citations?user=Qe9n2swAAAAJ&hl=en
Research gate ID- https://www.researchgate.net/profile/Mathew-John-7
Clinical Proteomics
Identifying the proteins involved in the inflammatory signaling cascade and their protein protein interactions (PPI) constitutes the central focus of our research. The discovery of previously unidentified proteins in blood samples will provide deeper insights into the downstream molecular signaling mechanisms underlying chronic inflammatory disease pathophysiology as well as discovery of newer biomarkers. Major diseases in focus are autoimmune diseases of neuronal and systemic origin, COPD, Ischemic stroke and Psychiatric disorders
Metabolomics
Metabolomics approach involves identifying lipid molecules involved in pathophysiology of autoimmune diseases and their utility in diagnostic and prognostic applications Phytochemistry
The Phytochemistry lab focuses on the extraction & characterization of secondary metabolites from tropical plants and evaluation of its biochemical effect using in vitro and in vivo models.
Ph.D. (CSIR fellowship), (2013) NIMHANS, Bangalore, Govt of India
M.Sc. Medical Biochemistry,(2006), JIPMER, Pondicherry, University, Govt of India
B.Sc. Biochemistry, (2002). Government College Kariavattom, (University of Kerala)
Scientist D (Jubilee Centre for Medical Research) April 2024 - Present
Scientist C (Jubilee Centre for Medical Research) May 2019 - Mar 2024
Scientist B (Jubilee Centre for Medical Research) June 2016 to Apr 2019
Tutor and Clinical Biochemist (2008 to 2009) Amala Institute of Medical Sciences, Thrissur
Postdoctoral Fellow (2013 to 2015)
Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram
Society of Biological Chemists ( India)
Proteomics Society of India
Reviewer of Elsevier , Springer Nature, Wiley and Taylor & Francis publications
CSIR Fellowship for PhD
DBT RA for Post Doctoral Fellowship
Ongoing
Projects
1. Area: Neuro Autoimmune Disease
Role: Principal Investigator
Funding Agency: Indian Council of
Medical Research(2023-2026)
Project Title: Lipid protein multi-omics
integrative approaches for differential diagnosis of autoimmune neuromyelitis
optica disease spectrum â a comparative cross sectional study
Budget: INR â36,00,000/-
2. Area: Breast Cancer
Role: Co- investigator (Protein Biology)
Funding Agency: ICMR, Govt. of India (2022-2025)
Project Title: Elucidating the association of
serum protein levels and genetic polymorphisms of FABP4 with obesity and breast
cancer risk in post-menopausal obese women.
Budget: INR â84,00,000 /-
Completed Projects
1. Area: Uterine Fibroid
Role: Co Investigator (Biochemical investigations )
Funding Agency: Directorate of Health Research (2021-2024)
Project Title: Non-invasive treatment of uterine fibroids through rationally
designed Selectiveprogesterone receptor modulators
Budget: INR â85,00,000/-
2026
J A, K K, George A, Prakash A, John M. Phytochemical characterization and biological evaluation of Carica papaya L. leaf alkaloids: In vitro and in silico insights into antioxidant, cytotoxicity, and anti-inflammatory activities. J Herbmed Pharmacol. 2026;15(1):66-74. doi: 10.34172/jhp.2026.5325
2025
Mohan V, Krishnan S, Balan S, Das S, Earali J, Maria E, Nair D, John M. Myosin Light Chain 12b and MASP1 as Novel Biomarker Candidates in Active Juvenile Idiopathic Arthritis Combined Proteomics/Bioinformatics Approach. Journal of Proteome Research. 2025 Feb 11.
Das S, Adiody S, Varghese J, John M. Relationship between autoimmunity and COPD: an investigation based on proteomic profiling and antinuclear antibody screening. Journal of Immunoassay and Immunochemistry. 2025 May 8:1-4.
Kumar SS, Remya C, Krishnakumar K, Maria E, Dileep KV, John M. Modulation of COX-2, 5-LOX, and cytokine signalling by Carica papaya L. Leaf cultivar Red Lady flavonoids in inflammation: in-vitro and in-silico insights. Natural Product Research. 2025 Sep 27:1-7.
Kumar SS, Prakash A, Keerthana PV, John M. 7-Ketocholesterol as a Critical Oxysterol: Impact on Human Health and Safety in Food Systems. The Journal of Steroid Biochemistry and Molecular Biology. 2025 Sep 5:106856.
2024
Das S, Varghese J, Adiody S, John M. Relevance of Trace Elements and Oxidative Stress in Chronic Obstructive Pulmonary Disease. Medical Journal of Dr. DY Patil University. 2024 Nov 1;17(Suppl 2):S353-60.
Das S, Adiody S, Varghese J, Vanditha M, Maria E, John M (Corresponding author). Exploring the novel duo of Reticulocalbin, and Sideroflexin as future biomarker candidates for Exacerbated Chronic Obstructive Pulmonary Disease. Clinical Proteomics. 2024 Dec;21(1):10
Kumar SS, Krishnakumar K, Maria E, John M (Corresponding author). Exploring the cytoprotective potential of flavonoid fraction from Carica papaya L. Cultivar Red Lady'Leaf: Insights into Nrf2 activation via in-vitro and in-silico approaches. South African Journal of Botany. 2024 Sep 1;172:373-87.
Das, S., John, M., & Adiody, S. (2024). Relevance of copper/zinc ratio and its association with redox status in chronic obstructive pulmonary disease. International Journal Of Community Medicine And Public Health, 11(6), 22912295. https://doi.org/10.18203/2394-6040.ijcmph20241487
Kumar SS, Gayathri TV, Krishnakumar K, John M (Corresponding author). Antioxidant potential and mineral elemental profiling of young and mature fruit and leaf of Carica papaya L. cultivar Red Lady Journal of Trace Elements and Minerals. 2024 May 31:100166.
2023
Das, S., John, M (Corresponding author). Maria, E. et al. A journey to unravel the pathophysiology of stable and exacerbated Chronic Obstructive Pulmonary Disease through erythrocyte proteomics: a combined mass spectrometry/bioinformatics approach. J Proteins Proteom 14, (2023). https://doi.org/10.1007/s42485-023-00120-w
Kumar SS, Krishnakumar K, John M (Corresponding author). Antihemolytic activity of flavonoids from butanolic extract of Carica papaya L. cultivarRed Ladyleaf. Food and Humanity. 2023 Dec 1;1:159-64.
Babu A, John M, Liji MJ, Maria E, Bhaskar SJ, Binukmar BK, Sajith AM, Reddy EK, Dileep KV, Sunil K. Sub-pocket-focused designing of tacrine derivatives as potential acetylcholinesterase inhibitors. Computers in Biology and Medicine. 2023 Feb 11:106666. https://doi.org/10.1016/j.compbiomed.2023.106666
2022
Kumar SS, Krishnakumar K, John M (Corresponding author). Flavonoids from the butanol extract of Carica papaya L. cultivar'Red Lady'leaf using UPLC-ESI-Q-ToF-MS/MS analysis and evaluation of the antioxidant activities of its fractions. Food Chemistry Advances. 2022 Oct 1;1:100126.
Maria E , Das S , Varghese An , Harisuthan T , and John M (corresponding author) Differentially Expressed Erythrocyte Proteins in Neuromyelitis Optica Spectrum Disorders and Their Functional Annotation Using DAVID Bioinformatics Tool. Neurochem J (2022) 16(4):465-71. (Springer Nature, Impact factor: 0.5)
Maria E, Yohannan M, Harisuthan T, John M (corresponding author) Comparative Proteomics of Lipid Transport Proteins and Assessment of Oxidative Stress Parameters in Acute Ischemic Stroke and Healthy Control. Int J of Biochem & Biophys. (2022),10(1):1-7
2021
John M (corresponding author) , Maria E, Das S. Identification of Novel Serum Proteins Associated with Myelination and Cholesterol Transport in Neuromyelitis Optica Spectrum Disorders by Mass Spectrometry. Indian Journal of Clinical Biochemistry. 2021 Nov 12:1-0.DOI: 10.1007/s12291-021-01004-w (Springer Nature, Impact factor: 1.6)
John M Priyanka S, George JB, Maria E, Unni G. Oxidative stress parameters in atherosclerotic cardiovascular disease high and low risk score groups as indicators of acute myocardial infarction. Int J Medical Sciences and Technology. 2021, 10(1):1-7 (EBSCO Indexed),
2019
Siddiqua MSH, John M, Manoj V.C, Santhakumar R. Cystatin C- an early marker indicative of renal dysfunction in critically ill children: a prospective cohort study. International J of Contemporary pediatrics. International J of Contemporary pediatrics. 2019; 6(5): 1981-84.
Kumar M, John M*, Madhavan M, James J, Omkumar RV. Alteration in the phosphorylation status of NMDA receptor GluN2B subunit by activation of both NMDA receptor and L-type voltage gated calcium channel. Neuroscience letters, 2019; 709 (14): 134343 * Equal contribution as first author
